Sunday, May 16, 2010

DNA Footprinting

bio technology Information


DNA Footprinting

DNA Footprinting was developed in 1977 and is an analytical procedure in molecular biology for identifying the specific sequence of DNA (the binding site) that binds to a particular protein. DNA Footprinting is most commonly performed on proteins that are thought to play some significant functional role such as gene regulation. This method can be performed on proteins which bind both double and single-stranded DNA. Additionally, DNA-binding proteins can be split into two groups, namely site-specific DNA-binding proteins and non-specific DNA–binding proteins.

DNA Footprinting uses a damaging agent such as a chemical reagent, radical or a nuclease that can cut or modify DNA at every base pair. However, where the ligand binds to DNA, the cleavage is restrained. DNA Footprinting discovers which specific parts of a DNA molecule have sites for specific proteins to attach to them. Using this technique, DNA that has first been in the presence of DNA-binding proteins and then exposed to a damaging agent, can be compared to DNA that was never exposed to the binding protein (and thus not protected against the damaging agent). The DNA sequence that is protected from cleaving can then be identified as the binding site.

DNA Footprinting can provide information that is, conceptually, much like fingerprinting in the sense that it can be used to identify a unique individual. DNA Footprinting can extract a banding pattern, or electropherogram, much like a bar code, that can identify a species or individual (some genes will be vary at the species level and others at the individual level)
this article abaout dna is frombtech-info.blogspot

1 comment:

  1. Hi there,

    Please help... I need to monitor a home made, 55 gln aerobic reactor with whole raw potato slurry, mineral complements and culture media... the idea is a SCP process to obtain nucleotide, amino and else nutrients.

    Will a regular educational fluorometer, turbidimeter or colorimeter with the right software do the work? Could you please suggest a software name and a low price optical device for this matter, including nucleotide, amino and else nutrients measuring?

    Thanks in advance, my best regards,

    Optical device for PH, and pO2 monitoring

    Fernando Ortiz Serrano
    Casa: (593) 42 391 213 Celular: (593) 92 363 544
    msn:arcaplan@hotmail.com skype:ecuafosy
    Guayaquil- Ecuador

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